Services

For your research needs, we offer metabolite profiling and absolute quantification of your metabolites by UHPLC-MS.

Due to the complexity of metabolomics experiments, we would like to meet you for discussing the details of your project. Once a strategy has been established, sample analysis will be scheduled. Please fill out and submit the Project information form. For further information or to schedule a meeting, please contact us.

Methods

The table below summarizes the LC-MS based analysis available in our facility. In addition, the Center of Laboratory Medicine offers over 350 routine analysis for quantifying metabolites in your samples (e.g., amino acid profile, organic acids profile). Prices are available on request.

Metabolic profile Matrix Type* Status
Non-targeted metabolic profile (C18 and HILIC) Plasma, serum, tissue, urine, cells Relative Available
Lipid profile (list available on request, e.g., LPS, PI, PC, PE, Cer, SM, CE, DG, TG) Plasma, serum Semi-quantitative Available
Targeted metabolic profile (110) Plasma, serum Relative Available
Acylcarnitine profile (28) Plasma, serum, dried blood spot Quantitative Available
Steroid profile (17) Plasma, serum Quantitative Available
Bile acids profile (16) Plasma, serum Relative Available
Amino acids profile Plasma Quantitative Available
Uracil and metabolites (dihydrouracil, β-ureidopropionic acid, β-alanine) Plasma Quantitative Available
Folic acid and metabolites Plasma Quantitative Coming soon

             

Therapeutic drugs & metabolites Matrix Type* Status
Sirolimus, Tacrolimus, Everolimus, Cyclosporin Plasma, tissue Quantitative Available
Timolol Humor Quantitative Available
Capecitabine and metabolites Plasma Quantitative Available
Octenidin Tissue Quantitative Available
Antifungal (7) Plasma, serum Quantitative Available
5-Fluorouracil and metabolites (5-fluorodihydrouracil, fluoro-β-ureidopropionic acid, fluoro-β-alanine) Plasma Quantitative Available
Rivaroxaban, Apixaban, Edoxaban, Edoxaban M4 Plasma Quantitative Available
Insulin & analogs Plasma Quantitative Available

       

Other Matrix Type* Status
7α-hydroxy-4-cholesten-3-one (bile acid precursor) Serum Quantitative Available
Albumin profile Plasma, serum Relative Available
Glycosaminoglycans Urine Quantitative Coming soon

*Type

  • Relative: fold change information are given by comparing the peak area of metabolites, no calibration curve is used
  • Semi-quantitative: concentration values are given based on a calibration curve obtained with a metabolite standard having similar physicochemical properties
  • Quantitative: concentration values are given based on a calibration curve obtained with authentic chemical standard of the metabolites. The method uses isotopically labeled metabolites and is usually validated